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CHRDL1 inhibits OSCC metastasis via MAPK signaling-mediated inhibition of MED29

Songkai Huang1, Junwei Zhang1, Yu Qiao2

  • 1School and Hospital of Stomatology, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou Medical University, Guangzhou, 510182, China.

Molecular Medicine (Cambridge, Mass.)
|October 27, 2024
PubMed
Abstract

Insights

The study reveals that MED29 promotes oral squamous cell carcinoma (OSCC) migration and invasion by facilitating epithelial-mesenchymal transition (EMT). CHRDL1 inhibits OSCC invasion and metastasis by suppressing MED29 and the MAPK signaling pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • The role of CHRDL1 in oral squamous cell carcinoma (OSCC) is largely unknown.
  • Investigating novel mRNA molecules like CHRDL1 is crucial for understanding cancer progression.

Purpose of the Study:

  • To elucidate the function of CHRDL1 in OSCC.
  • To explore the relationship between CHRDL1 and MED29 in OSCC progression.
  • To determine the underlying mechanisms of CHRDL1's action in OSCC.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) and immunohistochemistry to assess MED29 expression in OSCC tissues and cell lines.
  • Western Blot analysis for protein expression levels.
  • In vitro assays (scratch, Transwell, wound healing, immunofluorescence) to evaluate MED29 and CHRDL1 effects on OSCC cell migration, invasion, and epithelial-mesenchymal transition (EMT).
  • In vivo tail vein lung metastasis model in nude mice to assess CHRDL1's role in metastasis.

Main Results:

  • MED29 expression is upregulated in OSCC tissues and cell lines, correlating with increased migration and invasion.
  • MED29 overexpression promotes EMT, migration, and invasion in OSCC cells.
  • Knockdown of MED29 inhibits EMT, migration, and invasion.
  • CHRDL1 suppresses MED29 expression, thereby inhibiting EMT, invasion, and migration of OSCC cells.
  • CHRDL1 inhibits OSCC cell migration by regulating MED29.

Conclusions:

  • MED29 drives OSCC progression by promoting EMT, migration, and invasion.
  • CHRDL1 acts as an inhibitor of OSCC invasion and metastasis.
  • CHRDL1 exerts its inhibitory effects by suppressing MED29 expression, likely through the MAPK signaling pathway.
Keywords:
CHRDL1EMTMED29OSCC

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