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Related Concept Videos

Stability of Substituted Cyclohexanes02:30

Stability of Substituted Cyclohexanes

This lesson discusses the stability of substituted cyclohexanes with a focus on energies of various conformers and the effect of 1,3-diaxial interactions.
The two chair conformations of cyclohexanes undergo rapid interconversion at room temperature. Both forms have identical energies and stabilities, each comprising equal amounts of the equilibrium mixture. Replacing a hydrogen atom with a functional group makes the two conformations energetically non-equivalent.
For example, in...
Recrystallization: Solid–Solution Equilibria01:10

Recrystallization: Solid–Solution Equilibria

Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
Stability of Equilibrium Configuration01:23

Stability of Equilibrium Configuration

Understanding the stability of equilibrium configurations is a fundamental part of mechanical engineering. In any system, there are three distinct types of equilibrium: stable, neutral, and unstable.
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Stability of Equilibrium Configuration: Problem Solving01:13

Stability of Equilibrium Configuration: Problem Solving

The stability of equilibrium configurations is an important concept in physics, engineering, and other related fields. In simple terms, it refers to the tendency of an object or system to return to its equilibrium position after being disturbed. The stability of an equilibrium configuration can be analyzed by considering the potential energy function of the system and examining its behavior near the equilibrium point.
Problem-solving in the context of the stability of equilibrium configuration...

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Related Experiment Video

Updated: Jun 19, 2026

Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
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Modeling compound lipid homeostasis using stable isotope tracing.

Karl Wessendorf-Rodriguez, Maureen L Ruchhoeft, Ethan L Ashley

    Biorxiv : the Preprint Server for Biology
    |October 28, 2024
    PubMed
    Summary
    This summary is machine-generated.

    We developed compound lipid metabolic flux analysis (CL-MFA) to quantify lipid metabolism. This method reveals how liver kinase B1 (LKB1) affects sphingolipid recycling in non-small cell lung cancer (NSCLC).

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    Area of Science:

    • Metabolomics
    • Molecular Biology
    • Biochemistry

    Background:

    • Lipids are crucial for cellular functions, and their metabolism is implicated in diseases like cancer.
    • Existing tools for quantifying lipid metabolic fluxes are limited.
    • Understanding lipid homeostasis is vital for disease research.

    Purpose of the Study:

    • To develop and apply a novel method, compound lipid metabolic flux analysis (CL-MFA), for quantifying lipid metabolic fluxes.
    • To investigate liver kinase B1 (LKB1) regulation of sphingolipid metabolism in non-small cell lung cancer (NSCLC).
    • To analyze the impact of standard tissue culture conditions on lipid metabolism.

    Main Methods:

    • Stable isotope tracing.
    • Liquid chromatography-high-resolution mass spectrometry (LC-HRMS).
    • Network-based isotopologue modeling.

    Main Results:

    • CL-MFA enables concurrent quantification of fatty acid synthesis, elongation, headgroup assembly, and salvage reactions.
    • LKB1-mediated regulation of sphingolipid recycling in NSCLC cells and lung tissue was resolved.
    • Standard tissue culture conditions were found to induce supraphysiological fatty acid synthase flux.
    • Novel isozyme specificity of ceramide synthase inhibitors was identified.

    Conclusions:

    • CL-MFA is a powerful tool for quantifying lipid cycling and uncovering biological functions.
    • This approach elucidates molecular mechanisms in membrane lipid metabolism.
    • The findings provide insights into LKB1's role in NSCLC and the impact of culture conditions on lipid metabolism.