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Growing microglia in the lab

Jonathan M Levenson1, Hio Tong Kam2, Dong Feng Chen2

  • 1FireCyte Therapeutics, Inc, Boston, United States.

Elife
|October 28, 2024
PubMed

Keywords:
MicrogliaNaIO3PLX-5622RPEhuman iPSCmouseregenerative medicineretinal transplantationstem cells

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Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media