Persistent chemiluminescence-based nanosensor for portable point-of-care testing of H₂O₂

A novel glow-type chemiluminescence (CL)-based nanosensing system was developed for sensitive and rapid point-of-care testing (POCT) of H₂O₂ in food. CuSe nanoparticles (CuSe_NPs) have excellent peroxidase-like activity. After being modified with thiols of 4-mercaptophenylboronic acid (MBA) (CuSe_NPs@MBA), luminol can be catalyzed to produce long-lasting CL in the presence of H₂O₂. The possible reason for the long-lasting glow-type CL behavior was explored. Under the optimized condition, H₂O₂ can be sensitively detected with improved repeatability. The limit of detection is as low as 0.30 μM. To meet the requirement of in situ and outside of laboratory detection, a 3D-printed portable device was designed which can eliminate the environmental interference to improve detection accuracy. The developed multifunctional platform also has the advantages of simple operation and low cost, suggesting its great potential for applications in food and agricultural fields.