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Updated: Jun 9, 2025
Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates
Published on: March 5, 2019
Excellent properties of NaF and NaBr induced DNA/gold nanoparticle conjugation system: Better stability, shorter
Hai-Bo Wang1, Liang Zhang2, Tian-Yu Hu3
1Institute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Chuzhou, 233100, Anhui, China.
Abstract:
The functionalization of gold nanoparticle (AuNP) is the key procedure for the biochemical and biomedical application. The conventional salt-aging method requires the stepwise additions of NaCl and excessive thiolated DNA, mainly due to the poor tolerance of the DNA/AuNP mixture toward NaCl. Herein, we found that NaF is capable of improving the stability for the modification of AuNP with different bases of DNA sequences (poly A/T/C/G), and allows for adding up with a high concentration of 200 mM at one time, which greatly reduces the total modification time to 0.5-1 h. Intriguingly, the introduction of NaBr effectively increases the DNA loading capacity. Besides the advantages of NaF and NaBr, the modification performance is improved via the introduction of the oligo A/T spacer for the G-rich DNA sequences. Furthermore, this method shows the superiority to another two methods (pH 3-based and salt-aging) in terms of the loading capacity or sequence components.
