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Updated: Jun 9, 2025

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Selective bioorthogonal probe for N-glycan hybrid structures.

Mana Mohan Mukherjee1, Devin Biesbrock1, Lara K Abramowitz1

  • 1Laboratory of Cell and Molecular Biology, NIDDK, National Institutes of Health, Bethesda, MD, USA.

Nature Chemical Biology
|October 29, 2024
PubMed
Summary

Researchers developed a new chemical reporter, MM-JH-1, for selectively labeling hybrid N-glycans. This advancement enables precise tracking of proteins with hybrid N-glycans within cells.

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Area of Science:

  • Biochemistry
  • Chemical Biology
  • Glycobiology

Background:

  • Metabolic labeling with tagged monosaccharides allows for glycoprotein and glycolipid tagging.
  • Achieving selectivity in labeling is challenging due to shared monosaccharide precursor pathways.
  • N-linked glycosylation, a complex posttranslational modification, yields three main N-glycan structures: oligomannose, hybrid, and complex.

Purpose of the Study:

  • To develop a next-generation metabolic chemical reporter for selective labeling of hybrid N-glycan structures.
  • To establish a general strategy for defining the selectivity of chemically tagged monosaccharide labeling.
  • To utilize the reporter for imaging and fractionation to understand the localization and trafficking of hybrid N-glycan-bearing proteins.

Main Methods:

  • Synthesis of the novel chemical reporter 1,3-Pr2-6-OTs GlcNAlk (MM-JH-1).
  • Development of a general strategy to assess the selectivity of metabolic labeling.
  • Application of MM-JH-1 for selective labeling and subsequent cellular imaging and fractionation.

Main Results:

  • MM-JH-1 was successfully synthesized as a chemical reporter.
  • The study established that MM-JH-1 is selectively incorporated into hybrid N-glycans.
  • Imaging and fractionation experiments revealed the intracellular localization and trafficking of target proteins with hybrid N-glycans.

Conclusions:

  • MM-JH-1 serves as a valuable tool for the selective metabolic labeling of hybrid N-glycans.
  • This reporter facilitates the study of hybrid N-glycan-bearing proteins' cellular dynamics.
  • The developed strategy provides a framework for designing selective metabolic labeling probes.