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Spermatogenesis

Spermatogenesis

Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...

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Related Experiment Video

Updated: Jun 9, 2025

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

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Published on: March 4, 2018

Germ cell progression through zebrafish spermatogenesis declines with age.

Andrea L Sposato¹, Hailey L Hollins¹, Darren R Llewellyn¹

¹School of Biological Sciences, University of Utah, Salt Lake City, UT 84112, USA.

Development (Cambridge, England)

|October 29, 2024

Summary

Aging zebrafish testes show a decline in sperm production with age. Single-cell RNA sequencing revealed distinct molecular changes in spermatogonia and increased immune cells in older testes, impacting fertility.

Keywords:

Aging Immune cells Male fertility Single-cell RNA sequencing Spermatogenesis Testis

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Last Updated: Jun 9, 2025

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

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Published on: March 4, 2018

Dissection of Larval Zebrafish Gonadal Tissue

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Published on: April 26, 2017

Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

Published on: March 3, 2020

Area of Science:

Reproductive Biology
Developmental Biology
Aging Research

Background:

Spermatogonial stem cells are crucial for lifelong sperm production.
Fertility naturally declines with age, but the underlying mechanisms are unclear.
Morphological studies offer limited insight into age-related spermatogenesis decline.

Purpose of the Study:

To create a developmental atlas of the aging zebrafish testis using single-cell RNA sequencing.
To identify molecular and cellular changes associated with age-related decline in spermatogenesis.
To understand the regulation of spermatogenesis during aging.

Main Methods:

Single-cell RNA sequencing (scRNA-seq) was employed to analyze zebrafish testes across different ages.
Transcriptional profiles of individual cells were generated to build a developmental atlas.
Cellular composition and gene expression patterns were compared between young and aged testes.

Main Results:

A progressive decline in spermatogenesis was observed with increasing age in zebrafish.
Older testes showed reduced populations of spermatocytes and distinct transcriptional profiles in spermatogonia.
A significant increase in immune cells, including macrophages and lymphocytes, was noted in testes with impaired spermatogenesis.
The study identified age-correlated cellular and molecular alterations in the aging testis.

Conclusions:

The aging zebrafish testis undergoes significant cellular and molecular remodeling.
Distinct molecular signatures in aging spermatogonia and altered immune cell populations are linked to fertility decline.
This study provides a molecular roadmap for understanding and potentially intervening in age-related spermatogenesis dysfunction.