Multicomponent Resistance Responses of Melon to Zucchini Yellow Mosaic Virus

A major resistance gene of the melon accession PI414723 to zucchini yellow mosaic virus (ZYMV) is located at the Zym-1 locus on chromosome 2, but the underlying defense mechanisms are poorly understood. The physiological responses and expression of selected genes at Zym-1 were assessed in PI414723 and in the susceptible genotype Védrantais. Viral titers and the expression of genes related to systemic acquired resistance were evaluated in inoculated (Inoc) and noninoculated (Non-Inoc) portions of the cotyledons at 3, 7, and 10 days after inoculation and in apical leaves at 10 days after inoculation. ZYMV was detected in both portions of the cotyledons but not in the apical leaves of PI414723 plants. Also, ZYMV was recovered in a susceptible zucchini only from Inoc portions at 3 days after inoculation. By contrast, in Védrantais, ZYMV was detected and recovered from all tissues at high concentrations. Starchy local lesions and accumulation of transcripts of the systemic acquired resistance marker genes PR1 and PR4 were also detected in the resistant genotype. Plus, transcripts of one candidate resistance gene analog previously located at Zym-1 and of two melon homologs of restricted tobacco etch virus movement 2 genes located close to Zym-1 accumulated only in PI414723. It is proposed that resistance results from the combined action of the R gene involved in restricting ZYMV replication after a supposed recognition event and of the restricted tobacco etch virus movement genes that impact viral systemic movement to distal apical tissues.