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High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.
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Developing a model to implement marker-assisted selection for root-knot nematode resistance in common bean.

Talissa de Oliveira Floriani1,2, Henrique Castro Gama1, Bruna Marques Moreno1

  • 1Department of Genetics, 'Luiz de Queiroz' College of Agriculture, University of Sao Paulo, Piracicaba, SP 13418-900, Brazil.

G3 (Bethesda, Md.)
|November 9, 2025
PubMed
Summary

Common bean resistance to root-knot nematodes was investigated using genetic mapping. Four major quantitative trait loci were identified, aiding marker-assisted selection for improved crop resilience.

Keywords:
Meloidogyne incognitaPhaseolus vulgarisQTL mappingcandidate genes

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Area of Science:

  • Plant genetics
  • Agronomy
  • Nematology

Background:

  • Common bean (Phaseolus vulgaris L.) is crucial for global food security, providing essential nutrients and protein.
  • Root-knot nematodes, particularly Meloidogyne incognita, cause significant yield losses in common bean cultivation.
  • Developing resistant crop varieties is a primary strategy to manage nematode infections and ensure agricultural productivity.

Purpose of the Study:

  • To investigate the genetic basis of common bean resistance to race 3 of Meloidogyne incognita.
  • To identify quantitative trait loci (QTLs) associated with resistance traits, specifically the root-galling index.
  • To identify candidate genes for nematode resistance and facilitate marker-assisted selection.

Main Methods:

  • Controlled crosses were made between resistant and susceptible common bean genotypes (IAC-Tybatã and Branquinho).
  • A segregating F2 population (333 individuals) was genotyped using genotyping-by-sequencing (GBS).
  • Phenotypic data (egg mass, root-galling index, root dry mass) were collected from F2:3 families, followed by linkage and QTL mapping.

Main Results:

  • A linkage map with 954 SNPs across 11 linkage groups was constructed.
  • Four major QTLs associated with the root-galling index were identified on chromosomes Pv03, Pv05, Pv08, and Pv10.
  • Epistasis between QTLs on Pv08 and Pv10 was observed for the root-galling index; no significant QTLs were found for egg mass or root dry mass.

Conclusions:

  • The identified QTLs provide a foundation for marker-assisted selection to develop common bean varieties resistant to Meloidogyne incognita.
  • Candidate genes within the QTL intervals, particularly on Pv10, are implicated in nematode resistance.
  • This research contributes valuable genetic insights for breeding programs aimed at enhancing common bean's resilience against root-knot nematodes.