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Updated: Jun 9, 2025

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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Strategies for programmable manipulation of alternative splicing

Jonathan C Schmok1, Gene W Yeo2

  • 1Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA, USA; Sanford Stem Cell Institute Innovation Center and Stem Cell Program, University of California San Diego, La Jolla, CA, USA; Institute for Genomic Medicine, University of California San Diego, La Jolla, CA, USA; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

Current Opinion in Genetics & Development
|October 29, 2024
PubMed
Summary

This review covers four strategies for controlling alternative splicing (AS), including antisense oligonucleotides (ASOs), CRISPR, synthetic factors, and engineered RNAs. These methods offer precise RNA modulation for disease treatment and research.

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