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Bisphenol AP Exposure-Induced Inflammatory Response in Zebrafish Embryos Revealed by Transcriptomic Analysis
Qingjiang Ding1,2,3, Hailin Wang1,2,3
1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Environment & Health (Washington, D.C.)
|October 30, 2024
Summary
Bisphenol AP (BPAP), a BPA substitute, causes developmental toxicity in zebrafish embryos. Exposure led to abnormal development and induced inflammatory responses, highlighting environmental and health risks.
Area of Science:
- Environmental Science
- Toxicology
- Developmental Biology
Background:
- Bisphenol AP (BPAP) is increasingly used as a substitute for Bisphenol A (BPA).
- BPAP is prevalent in the environment, but its developmental toxicity in aquatic organisms is poorly understood.
- Zebrafish embryos are a valuable model for assessing early life-stage toxicity.
Purpose of the Study:
- To investigate the developmental toxicity of Bisphenol AP (BPAP) in zebrafish embryos.
- To determine the lethal concentrations of BPAP at different embryonic developmental stages.
- To explore the molecular mechanisms underlying BPAP-induced toxicity.
Main Methods:
- Exposure of zebrafish embryos to varying concentrations of BPAP.
- Assessment of embryonic survival rates and developmental abnormalities (hatching, malformation, movement).
- Transcriptomic analysis to identify gene expression changes, focusing on inflammatory pathways.
Main Results:
- Median lethal concentrations (LC50) of BPAP were determined at 24, 48, and 72 hours post fertilization (hpf).
- BPAP exposure caused significant developmental issues, including delayed hatching, physical malformations, and reduced spontaneous movement.
- Upregulation of inflammatory marker genes (il1b, ptgs2b, fosab) was observed, indicating an inflammatory response.
Conclusions:
- BPAP exhibits significant developmental toxicity in zebrafish embryos.
- BPAP exposure induces inflammatory responses at the molecular level.
- Findings highlight potential environmental and health risks associated with BPAP exposure.
