Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence specific antibodies to a deoxyribodinucleotide.

S C Chandrasekharappa, A S Gopalakrishnan, T M Jacob

    Biochemistry International
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Re: COVID-19: could CT provide the best population level biomarker? Incidental COVID-19 in major trauma patients suggests higher than predicted rates of infection in London. A reply.

    Clinical radiology·2021
    Same author

    COVID-19: using chest CT of major trauma patients to monitor and evaluate the effect of lockdown and the importance of household size.

    Clinical radiology·2021
    Same author

    COVID-19: could CT provide the best population level biomarker? Incidental COVID-19 in major trauma patients suggests higher than predicted rates of infection in London.

    Clinical radiology·2020
    Same author

    Neuroanatomic, epigenetic and genetic differences in monozygotic twins discordant for attention deficit hyperactivity disorder.

    Molecular psychiatry·2017
    Same author

    The primacy of NF1 loss as the driver of tumorigenesis in neurofibromatosis type 1-associated plexiform neurofibromas.

    Oncogene·2017
    Same author

    Somatic mutational landscape of AML with inv(16) or t(8;21) identifies patterns of clonal evolution in relapse leukemia.

    Leukemia·2015
    Same journal

    Monitoring of haemoglobin-methyl isocyanate adduct by high-performance liquid chromatography with diode array detector.

    Biochemistry international·1992
    Same journal

    Intervention of adriamycin induced free radical damage.

    Biochemistry international·1992
    Same journal

    Relationship between substrate activity and pKa value of phenols on sulfotransferase from Eubacterium A-44.

    Biochemistry international·1992
    Same journal

    Purification and properties of chitinase from cabbage.

    Biochemistry international·1992
    Same journal

    Coordinate expression of the enzymes of nitric oxide metabolism in Paracoccus denitrificans.

    Biochemistry international·1992
    Same journal

    Characterization of trehalase in Rhodotorula rubra.

    Biochemistry international·1992
    See all related articles

    Researchers developed specific antibodies targeting the dinucleotide deoxyribotetranucleotide phosphate A (dpTpA). These antibodies recognize dpTpA within DNA sequences, offering a precise tool for molecular biology research.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Biochemistry

    Background:

    • Development of specific antibodies is crucial for identifying and studying particular nucleic acid sequences.
    • The dinucleotide deoxyribotetranucleotide phosphate A (dpTpA) is a specific DNA sequence of interest.

    Purpose of the Study:

    • To elicit and characterize antibodies specifically binding to the dinucleotide dpTpA.
    • To determine the sequence specificity and binding characteristics of the generated antibodies.
    • To explore the utility of these antibodies in detecting dpTpA sequences within DNA.

    Main Methods:

    • Antibody generation in rabbits against dpTpA conjugated to a carrier protein.
    • Nitrocellulose filter binding assays to test antibody binding to various oligonucleotides.

    Related Experiment Videos

  • Affinity chromatography for antibody purification.
  • Competition studies using labeled and unlabeled oligonucleotides to assess binding specificity.

    • Main Results:

    • Antibodies specifically bound to dpTpA and oligonucleotides containing this sequence.
    • Binding inhibition studies confirmed the antibodies' specificity for the entire dpTpA molecule.
    • Purified antibodies demonstrated binding to denatured DNA at dpTpA sequences.
    • Antibodies did not exhibit binding to RNA.

    Conclusions:

    • Successfully generated sequence-specific antibodies targeting the dinucleotide dpTpA.
    • These antibodies are valuable tools for detecting dpTpA sequences in denatured DNA.
    • The findings open avenues for further research into the biological roles of dpTpA motifs in DNA.