Fatty acid biosynthesis in the peripheral nervous system of normal and Trembler mice

De novo fatty acid biosynthesis was demonstrated in a particle-free supernatant from normal and Trembler mouse sciatic nerves. In both systems, it required acetyl-CoA and malonyl-CoA, and led chiefly to the formation of free palmitic acid. No palmitoyl-CoA formation was detected. The ability of the cell-free extract of the mutant to form palmitic acid in vitro was greater than that of the control extracts when the results were expressed as the total activity per 100 mg of freshly excised sciatic nerves.