Preparation and characterization of polysaccharide-based conductive hydrogels for nerve repair

Peripheral nerve injury is a serious medical condition, but the limited availability of autologous grafts often delays timely treatment for many patients. The implantation of functional hydrogels with good biocompatibility provides an effective solution to this challenge. In this study, a novel chitosan derivative, choline functionalized catechol carboxymethyl chitosan (CF-Catechol-CMCS), was synthesized by modifying carboxymethyl chitosan (CMCS) with choline functionalized (CF) molecule and catechol. CF-Catechol-CMCS, sodium alginate (SA), and pyrrole monomer (Py) were then combined, crosslinked with Fe³⁺ and Ca²⁺, and polymerized in situ to form polypyrrole (PPy), resulting in the CF-Catechol-CMCS/SA/PPy hydrogel. This hydrogel exhibits excellent thermal stability, with a maximum thermal degradation temperature of 580 °C. By adjusting the ratio of PPy to CF-Catechol-CMCS/SA in the hydrogel, its degradation properties, swelling behavior, mechanical properties, and electrical conductivity can be fine-tuned. Specifically, when the mass ratio of PPy to CF-Catechol-CMCS/SA is 8:10, the hydrogel achieves optimal conductivity within a safe range (1.82 × 10^-3 S·cm^-1). By controlling the mass ratio of CF-Catechol-CMCS to SA in the hydrogel, the acetylcholine concentration can be regulated. When the CF-Catechol-CMCS:SA ratio is 2:1, the measured Sciatic Function Index (SFI) value is -34.54, indicating that the CF-Catechol-CMCS/SA/PPy hydrogel has excellent nerve repair potential.