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Related Concept Videos

The Bronchial Tree01:23

The Bronchial Tree

The human bronchi and bronchial tree play a crucial role in the respiratory system, facilitating the exchange of oxygen and carbon dioxide. Let's delve into the intricate structure and functions of these respiratory components.
The trachea, commonly known as the windpipe, is a tube that connects the larynx (voice box) to the bronchi. At a point called the carina, it bifurcates into two primary bronchi. The right primary bronchus is wider, shorter, and more vertical than the left primary...

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High-throughput Bronchus-on-a-Chip system for modeling the human bronchus.

Akina Mori1, Marjolein Vermeer2, Lenie J van den Broek2

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|November 1, 2024
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Summary

A new Bronchus-on-a-Chip system mimics human airways to study chronic airway inflammation and mucus production, offering a more accurate in vitro model for diseases like COPD and asthma.

Keywords:
3D-reconstructed airway epithelial cellsAirway-on-a-chipOrganotypic culture

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Area of Science:

  • Biomedical Engineering
  • Respiratory Medicine
  • Cell Biology

Background:

  • Chronic airway inflammation, seen in COPD and asthma, involves goblet cell hyperplasia and metaplasia, leading to airflow obstruction.
  • Current in vitro models using horizontal cell cultures do not accurately replicate the human airway's tubular structure or airway stenosis.
  • A need exists for advanced in vitro systems that better model human airway physiology for studying chronic inflammatory responses.

Purpose of the Study:

  • To develop and validate a novel Bronchus-on-a-Chip (BoC) system for studying chronic airway inflammation.
  • To create a more physiologically relevant in vitro model that replicates the human bronchial epithelium's tubular structure and cellular responses.
  • To establish a high-throughput platform for assessing mucus hyperproduction and other chronic epithelial responses.

Main Methods:

  • A microfluidic Bronchus-on-a-Chip (BoC) system was designed, allowing for the culture of 62 chips in a standard plate.
  • Human bronchial epithelial cells were cultured on a collagen extracellular matrix within a tubular structure for up to 35 days.
  • The system was characterized using barrier integrity assays, microscopy, histological examination, and exposure to inducers of goblet cell hyperplasia and metaplasia.

Main Results:

  • The BoC system successfully cultured human bronchial epithelial cells in a tubular, air-lifted configuration, mimicking native airway structure.
  • Epithelial cells differentiated into basal, ciliated, and secretory cell types, consistent with human bronchial epithelium.
  • Exposure to inflammatory inducers resulted in mucus hyperproduction, accurately replicating chronic epithelial responses observed in diseases.

Conclusions:

  • The developed Bronchus-on-a-Chip system provides a more human-relevant and accurate in vitro model for studying bronchial inflammation.
  • This system enhances the assessment of chronic cell responses, including mucus hyperproduction, relevant to COPD and asthma.
  • The BoC offers a high-throughput platform for advancing research into airway diseases and potential therapeutic interventions.