Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ca2+ induced phase separations in phospholipid mixtures.

W Tamura-Lis, E J Reber, B A Cunningham

    Chemistry and Physics of Lipids
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    <i>Mycobacterium tuberculosis</i> genotypes isolated from clinical specimens of extrapulmonary TB.

    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2025
    Same author

    Behavioral, metabolic, and lipidomic characterization of the 5xFADxTg30 mouse model of Alzheimer's disease.

    iScience·2024
    Same author

    The impact of the COVID-19 pandemic on UK medical education. A nationwide student survey.

    Medical teacher·2021
    Same author

    Clinical outcomes among patients with tuberculous meningitis receiving intensified treatment regimens.

    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2021
    Same author

    Highly Variable Expression of <i>ESR1</i> Splice Variants in Human Liver: Implication in the Liver Gene Expression Regulation and Inter-Person Variability in Drug Metabolism and Liver Related Diseases.

    Journal of molecular and genetic medicine : an international journal of biomedical research·2020
    Same author

    The blueprint for prevention of nosocomial tuberculosis transmission is clear, but why don't we have the will to follow it?

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2020
    Same journal

    The impact of avobenzone and oxybenzone on fibroblast and keratinocyte membranes. Searching for the role of lipids in the effect of UV filters on skin cells.

    Chemistry and physics of lipids·2026
    Same journal

    Interaction of tryptophan-based peptides with mixed lipid bilayers modulates bilayers' hydrophobic region in an anionic lipid-dependent manner.

    Chemistry and physics of lipids·2026
    Same journal

    Emerging roles of dehydrogenase/reductase (DHRS) in cancer.

    Chemistry and physics of lipids·2026
    Same journal

    Molecular dynamics insights into lipid bilayer electroporation under constant versus pulsed DC electric fields.

    Chemistry and physics of lipids·2026
    Same journal

    7-ketocholesterol as a theranostic target: Potential applications and future perspectives.

    Chemistry and physics of lipids·2026
    Same journal

    Corrigendum to Lipid membrane behavior of nitro-fatty acids and their loading into liposomes to activate Nrf2 pathway in RAW264.7 cells with impact on intracellular NO production [Chem. Phys. Lipids 270 (2025) 105497].

    Chemistry and physics of lipids·2026
    See all related articles

    Phase separation in lipid mixtures containing phosphatidylcholines (PC) and phosphatidylethanolamines (PE) was investigated. The study found that mixtures of two different lipids are necessary for phase separation in CaCl2 solutions, not individual lipid types.

    Area of Science:

    • Biochemistry
    • Physical Chemistry
    • Materials Science

    Background:

    • Phosphatidylcholines (PC) and phosphatidylethanolamines (PE) are key components of biological membranes.
    • Calcium chloride (CaCl2) solutions are known to influence lipid phase behavior.
    • Previous studies observed phase separation in specific lipid types within CaCl2 solutions.

    Purpose of the Study:

    • To investigate the phase separation behavior of mixed lipid systems (PC and PE) in CaCl2 solutions.
    • To determine if individual lipid species or lipid mixtures are responsible for phase separation.
    • To elucidate the role of lipid composition in CaCl2-induced phase separation.

    Main Methods:

    • Preparation of lipid mixtures including egg yolk phosphatidylethanolamine (EYPE), dioleoylphosphatidylcholine/dioleoylphosphatidylethanolamine (DOPC/DOPE), and palmitoyloleoyl-PC.

    Related Experiment Videos

  • Exposure of lipid mixtures to CaCl2 solutions.
  • Observation and analysis of phase separation phenomena.

    • Main Results:

    • EYPE and a 1:1 molar ratio of DOPC/DOPE mixtures exhibited phase separation in CaCl2 solutions.
    • Palmitoyloleoyl-PC, a mixed-chain lipid, did not show phase separation in water or CaCl2 solution.
    • Phase separation was observed in lipid mixtures but not in single-component systems.

    Conclusions:

    • The presence of at least two distinct lipid species is required for the observed phase separation in CaCl2 solutions.
    • Phase separation is a characteristic of the lipid mixture, rather than a property of individual lipid types.
    • This finding contributes to understanding the complex phase behavior of membrane lipids in the presence of divalent cations.