Promoting role of nitrogen-fixing bacteria and biochar on nitrogen retention and degradation of PBAT plastics during...

Since the increasing number of polybutylene adipate terephthalate (PBAT)-based plastics entering the environment, the search for sustainable treatment methods has become a primary focus of contemporary research. Composting offers a novel approach for managing biodegradable plastics. However, a significant challenge in the composting process is how to control nitrogen loss and enhance plastic degradation. In this context, the effect of various additives on nitrogen retention, PBAT plastics degradation, and microbial community dynamics during composting was investigated. The findings revealed that the addition of nitrogen-fixing bacteria Azotobacter vinelandii and biochar (AzBC) significantly improved nitrogen retention and accelerated PBAT rupture within 40 days of composting. Specifically, the PBAT degradation rate in the AzBC group reached 29.6%, which increased by 12.14% (P < 0.05) compared to the control group. In addition, the total nitrogen (TN) content increased by 6.20% (P < 0.05), and the Nitrogen-fixing enzyme (NIT) content increased by 190 IU/L (P < 0.05). Further analysis of GC-MS confirmed the presence of low molecular weight fragmentation products, such as 6-(4-hydroxybutoxy)-6-oxohexanoic acid. The AzBC treatment promoted the proliferation of Klebsiella at the genus level that could enhance nitrogen retention and the bacteria that have the ability to degrade PBAT, such as Proteobacteria and Firmicutes at the phyla level, and Pseudoxanthomonas, Pseudomonas, and Flavobacterium genera at the genera level (P < 0.05). Correlation analysis indicated that the degradation of PBAT is positively correlated with Temperature (T), NIT, and TN, but negatively correlated with the organic matter (OM) content and germination index (GI). In conclusion, the co-application of biochar and Azotobacter vinelandii offers promising sustainable prospects for enhancing PBAT plastic degradation and reducing nitrogen loss during composting.