Supercritical Fluid Chromatography in Bioanalysis-A Review.

Area of Science:

• Analytical Chemistry
• Bioanalysis

Background:

• Supercritical fluid chromatography (SFC) has seen significant improvements in instrumentation and hyphenation with mass spectrometry (MS) over the last decade.
• These advancements have led to increased acceptance and application of SFC in analytical chemistry, particularly in bioanalysis.

Purpose of the Study:

• To review the advantages and main applications of SFC in bioanalysis over the past 5 years.
• To discuss fundamental aspects including mobile phase composition, stationary phase selection, MS hyphenation, and matrix effects.

Main Methods:

• Review of recent literature on SFC applications in bioanalysis.
• Discussion of key technical considerations for SFC-MS in biological sample analysis.

Main Results:

• SFC offers unique selectivity, rapid analysis times, reduced organic solvent consumption, and a greener mobile phase.
• Increased publications highlight SFC's growing role in bioanalysis.

Conclusions:

• SFC is a valuable and increasingly utilized tool in bioanalysis.
• Understanding mobile phase, stationary phase, MS hyphenation, and matrix effects is crucial for successful SFC applications in bioanalysis.