Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

56.9K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
56.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hemangiosarcoma: A Systematic Review of Biomarkers in Diagnosis, Prognosis, and Therapeutic Strategies.

Veterinary and comparative oncology·2026
Same author

Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same author

Equine endometrial organoids preserve tissue structure and cycle-stage transcriptional identity†.

Biology of reproduction·2025
Same author

When assessing representative images of fine-needle aspirate cytological smears of canine mast cell tumours, interobserver agreement is influenced by educational attainment.

The Journal of small animal practice·2025
Same author

The Poly (ADP-Ribose) Polymerase-1 Enzyme Is Overexpressed in Various Solid Canine Tumours: An Immunohistochemical Study.

Veterinary and comparative oncology·2025
Same author

Matrix degradation enhances stress relaxation, regulating cell adhesion and spreading.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Jun 8, 2025

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification
04:43

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification

Published on: October 11, 2024

1.8K

Digital PCR in Virology: Current Applications and Future Perspectives.

David Gleerup1,2, Wim Trypsteen2,3, Stephanie I Fraley4

  • 1Laboratory of Veterinary Morphology, Faculty of Veterinary Medicine, Ghent University, Campus Merelbeke, Salisburylaan 133, 9820, Merelbeke, Belgium.

Molecular Diagnosis & Therapy
|November 2, 2024
PubMed
Summary

Digital PCR (dPCR) offers superior precision and sensitivity for virology applications compared to quantitative PCR (qPCR). Innovations are enhancing its multiplexing capabilities to address cost and throughput limitations for future applications.

More Related Videos

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
07:54

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

4.5K
DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

679

Related Experiment Videos

Last Updated: Jun 8, 2025

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification
04:43

Author Spotlight: Optimizing Digital Droplet PCR Method for Accurate Adeno-Associated Viral Genome Quantification

Published on: October 11, 2024

1.8K
Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
07:54

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

4.5K
DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

679

Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • Digital PCR (dPCR) has become a significant tool in virology, with microfluidic innovations driving its increased adoption.
  • dPCR is recognized for outperforming quantitative PCR (qPCR) in key parameters like precision, sensitivity, accuracy, and inhibitor resistance.
  • Current applications include quantification, mutation detection, and analysis of environmental DNA and RNA samples.

Purpose of the Study:

  • To review the strengths and weaknesses of dPCR, particularly in the context of virology.
  • To explore current and future applications of dPCR in virology.
  • To discuss technological advancements like real-time dPCR and digital high-resolution melting.

Main Methods:

  • Review of existing literature and technological advancements in digital PCR.
  • Analysis of dPCR performance metrics compared to quantitative PCR.
  • Exploration of multiplexing capabilities and their impact on throughput and cost.

Main Results:

  • dPCR demonstrates superior performance over qPCR in precision, sensitivity, accuracy, repeatability, and inhibitor resistance.
  • High-throughput applications, such as during the SARS-CoV-2 pandemic, are still limited by cost and throughput.
  • Multiplexing in dPCR offers potential for simultaneous multi-target quantification, addressing throughput and cost challenges.

Conclusions:

  • Digital PCR is a powerful technique in virology, offering significant advantages over traditional qPCR.
  • Further development in multiplexing and emerging technologies like real-time dPCR and digital high-resolution melting are crucial for broader adoption.
  • Addressing cost and throughput limitations will unlock the full potential of dPCR in large-scale virological studies and diagnostics.