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Author Spotlight: Examining Volatile Sex Pheromone Influence on Male C. elegans Behavior
Published on: August 9, 2024
Fractionation of a sex-inducing substance from flatworms using open-column chromatography and reverse-phase
Kiyono Sekii1, Taro Watanabe2, Riku Ito2
1Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan; Faculty of Business and Commerce, Keio University, Hiyoshi 4-1-1, Yokohama, Kanagawa 223-8521, Japan.
Abstract:
A substance that sexualizes planarians, an ancestral group of parasitic flatworms, is widely present in planarians and parasitic flatworms. Here, we present a protocol for extracting and purifying the active fraction with sex-inducing activity. We describe steps for homogenization of flatworms, sample concentration, open-column chromatography, and reverse-phase high-performance liquid chromatography. We then detail a feeding bioassay to confirm sex-inducing activity. The obtained active fraction may positively affect parasitic flatworm sexual maturation and can be tested by adding it into the culture media. For complete details on the use and execution of this protocol, please refer to Sekii et al. (2023).1.
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