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Exploring the anatomy of Linguatula serrata using micro-computed tomography
Alice Birckhead1, Ryan O'Hare Doig2, Ann Carstens1,3
1School of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, Australia.
International Journal for Parasitology. Parasites and Wildlife
|November 5, 2024
Summary
Micro-computed tomography (micro-CT) provides detailed 3D anatomical insights into the zoonotic parasite Linguatula serrata. This non-destructive imaging reveals internal structures, aiding in parasite morphology studies.
Area of Science:
- Parasitology
- Veterinary Medicine
- Medical Imaging
Background:
- Micro-computed tomography (micro-CT) is a valuable non-destructive imaging technique for analyzing morphology and host-parasitic interactions.
- Linguatula serrata is a zoonotic pentastome parasite affecting dogs and ruminants globally, with limited recent descriptions of its internal anatomy.
Purpose of the Study:
- To describe the internal and external anatomy of adult Linguatula serrata using micro-CT.
- To compare the efficacy of phosphotungstic acid (PTA) and iodine (I2) staining for micro-CT analysis of L. serrata.
Main Methods:
- Ten adult L. serrata specimens were examined using micro-CT after fixation in 70% ethanol.
- Specimens were stained with either 0.3% PTA or 1% iodine (I2) to assess tissue contrast.
Main Results:
- Both PTA and I2 stains provided good tissue contrast, clearly visualizing external features like hooks, mouth, and buccal cadre.
- Virtual sections and 3D reconstructions enabled detailed visualization of the coelomic cavity, digestive tract, nervous system, and reproductive organs.
Conclusions:
- Micro-CT is effective for detailed anatomical description of L. serrata, including internal structures previously poorly documented.
- The generated images and descriptions serve as a crucial anatomical reference for L. serrata, supporting future research and diagnostics.
