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Related Experiment Video

Updated: May 20, 2026

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Published on: May 17, 2017

Developmental Imaging of Radish Sprouts Using Dynamic Optical Coherence Tomography.

Yiheng Lim¹, Shumpei Kojima¹, Pradipta Mukherjee^1,2

¹Computational Optics Group, University of Tsukuba, Tsukuba, Japan.

Journal of Biophotonics

|November 5, 2024

Summary

Dynamic optical coherence tomography (OCT) non-invasively visualizes radish sprout germination. High OCT signals in early growth indicate active water and nutrient transport, decreasing as tissues mature.

Keywords:

botany dynamic optical coherence tomography optical coherence tomography plant germination plant imaging

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Last Updated: May 20, 2026

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Published on: May 17, 2017

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RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

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Published on: August 8, 2017

Area of Science:

Plant Biology
Biophotonics
Non-invasive Imaging

Background:

Understanding plant germination is crucial for agriculture and basic science.
Traditional methods for observing germination can be invasive or lack cellular detail.
Optical Coherence Tomography (OCT) offers high-resolution cross-sectional imaging.

Purpose of the Study:

To investigate the germination process of radish sprouts using dynamic OCT.
To visualize cellular and tissue activities during radish sprout development non-invasively.
To correlate dynamic OCT signals with physiological processes like water and nutrient transport.

Main Methods:

Volumetric dynamic optical coherence tomography (OCT) was employed.
Sequential acquisition of 16 OCT images per scan.
Temporal variance analysis of each pixel to assess tissue dynamics.
Longitudinal observation of radish sprouts for up to 12 days.

Main Results:

Dynamic OCT successfully visualized radish sprout morphology and internal activities.
High dynamic OCT signals were observed in vessels and growing roots during early germination.
Signal intensity decreased as radish sprout tissues matured.
Dynamic OCT patterns reflected changes in water and nutrient transport.

Conclusions:

Dynamic OCT is a sensitive tool for studying plant germination and growth.
The technique can non-invasively monitor water and nutrient transport in developing plants.
Dynamic OCT provides insights into cellular activities related to plant development.