Developmental Imaging of Radish Sprouts Using Dynamic Optical Coherence Tomography
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Updated: May 20, 2026
A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development
Published on: May 17, 2017
Yiheng Lim1, Shumpei Kojima1, Pradipta Mukherjee1,2
1Computational Optics Group, University of Tsukuba, Tsukuba, Japan.
Abstract:
The germination process of radish sprouts was investigated in detail using volumetric dynamic optical coherence tomography (OCT). Dynamic OCT involves the sequential acquisition of 16 OCT images and subsequent temporal variance analysis of each pixel, enabling non-invasive visualization of the cellular and tissue activities of plants. The radish sprouts were longitudinally investigated for up to 12 days, and changes in morphology and dynamic OCT image patterns were observed as the plants developed. The dynamic OCT signals in the vessels and growing roots were relatively high in the early stage of germination and decreased as the tissue matured. These results suggest that dynamic OCT is sensitive to water and nutrient transport as well as cellular activities associated with plant growth.
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