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Updated: Jun 8, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
Published on: December 5, 2016
Highly sequence-specific, timing-controllable m6A demethylation by modulating RNA-binding affinity of m6A erasers
Kenko Otonari1, Yuri Asami1, Kosuke Ogata2
1Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. imiki@scl.kyoto-u.ac.jp.
Abstract:
Recent advancements in tools using programmable RNA binding proteins and m6A-erasers enable sequence-selective and timing-controllable m6A demethylation. However, off-target effects are still a concern. This study addresses the problem by reducing the RNA-binding ability of m6A-erasers. The modulated m6A-erasers achieved sequence-specific and timing-controllable m6A demethylation with minimal off-target activity.
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