RNA Extraction from Rice Immature Embryo Using Laser Capture Microdissection

Laser capture microdissection (LCM) enables the selective isolation of organs, tissues, and cells from surrounding tissues. Total RNA extracted from small tissue sections can be used for a variety of subsequent analysis such as RNA-seq analysis. Here, we describe a method for isolating embryos from rice ovary sections using LCM and extracting total RNA. We successfully obtained sufficient amount of total RNA from a single rice embryo at the globular embryo stage for RNA-seq analysis.