Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bowel Stenosis Following Immunotherapy in dMMR Colorectal Cancer: A Case Series.

Clinical colorectal cancer·2026
Same author

Extracellular matrix from decellularized porcine organs as scaffolds for insulin-secreting cells and pancreatic islets.

Frontiers in endocrinology·2026
Same author

Production and Formulation of Alcanivorax borkumensis SK2 Cell Powders for Marine Oil Spill Bioremediation.

Biotechnology and applied biochemistry·2025
Same author

Canadian Consensus Recommendations for Predictive Biomarker Testing in Gastric and Gastroesophageal Junction Adenocarcinoma.

Current oncology (Toronto, Ont.)·2024
Same author

In Vitro and In Vivo Biocompatibility of Bacterial Cellulose.

Journal of biomedical materials research. Part B, Applied biomaterials·2024
Same author

Malignant duodenal gastrointestinal neuroectodermal tumor (GNET): Case report and review of the literature.

International journal of surgery case reports·2024

Related Experiment Video

Updated: Jul 13, 2026

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
11:30

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

23.8K

Characterization of Extracellular Matrix Derived From Porcine Organs Decellularized Using Different Methods.

Vignesh Dhandapani1,2, Pakindame Boabekoa1,2, Martin Borduas3

  • 1Laboratoire de Bio-ingénierie et de Biophysique de l'Université de Sherbrooke, Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, Québec, Canada.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|November 6, 2024
PubMed
Summary

This study characterizes extracellular matrices (ECMs) from decellularized porcine organs. Different methods yield ECMs with varying protein content and collagen structure, crucial for regenerative medicine applications.

Keywords:
collagensextracellular matrix (ECM)glycosaminoglycans (GAGs)lamininmass spectrometry and proteomicstissue and organ decellularization

More Related Videos

Novel Process for 3D Printing Decellularized Matrices
08:14

Novel Process for 3D Printing Decellularized Matrices

Published on: January 7, 2019

7.0K
Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels
06:12

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels

Published on: December 8, 2023

1.4K

Related Experiment Videos

Last Updated: Jul 13, 2026

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
11:30

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

23.8K
Novel Process for 3D Printing Decellularized Matrices
08:14

Novel Process for 3D Printing Decellularized Matrices

Published on: January 7, 2019

7.0K
Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels
06:12

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels

Published on: December 8, 2023

1.4K

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Tissue Engineering

Background:

  • Regenerative medicine aims to create tissues and organs, potentially solving organ transplantation shortages.
  • The extracellular matrix (ECM) is vital, providing a scaffold and biochemical signals for cell growth and differentiation.
  • ECM composition includes fibrous proteins and proteoglycans, offering a dynamic microenvironment for cellular processes.

Purpose of the Study:

  • To characterize extracellular matrices (ECMs) derived from porcine organs using four distinct decellularization techniques.
  • To evaluate the impact of decellularization methods on ECM ultrastructure, composition, and residual cellular material.
  • To establish a multimodal approach for assessing ECM quality for regenerative medicine.

Main Methods:

  • Decellularization of porcine organs using four different methods.
  • Histological analysis (Hematoxylin and eosin) to assess cellular removal and ECM integrity.
  • Biochemical assays including DNA quantification, Bicinchoninic acid (BCA) assay for protein content.
  • Mass spectrometry and proteomic analysis for detailed protein composition.
  • Polarization microscopy to examine collagen fiber orientation.

Main Results:

  • Histology confirmed the absence of nuclei and presence of glycosaminoglycans (GAGs) and collagen in ECMs.
  • Native pancreas showed necrosis, impacting ECM quality, with reduced dsDNA content.
  • Mass spectrometry confirmed significant differences in protein composition (up to 2700 proteins) based on decellularization technique.
  • Residual double-stranded DNA (dsDNA) content in ECMs was lower than in native organs.
  • BCA assay indicated varying protein content across different organs and techniques.
  • Polarization microscopy revealed differences in collagen fiber orientation.

Conclusions:

  • Decellularization techniques significantly influence the resulting ECM's protein composition and structural integrity.
  • A multimodal characterization approach is essential for understanding ECM properties derived from different decellularization methods.
  • Optimizing decellularization is key to balancing cellular component removal with the preservation of ECM ultrastructure and composition for regenerative applications.