Related Experiment Video
Updated: Jun 8, 2025
03:52
Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse
Published on: March 2, 2018
9.8K
A Novel Delivery Approach of Clinical Inner Ear Gene Therapy
Summary
This study demonstrates a new method for inner ear gene therapy delivery using trans-round window membrane (RWM) microinjection via transcanal endoscopic ear surgery (TEES). This technique proved feasible for successful clinical application.
Area of Science:
- Otolaryngology
- Gene Therapy
- Surgical Innovation
Background:
- Developing effective delivery methods for inner ear gene therapy is crucial.
- Trans-round window membrane (RWM) delivery is a promising route for inner ear therapeutics.
Purpose of the Study:
- To develop and assess a novel trans-RWM microinjection technique for inner ear gene therapy.
- To optimize the use of a pipetting microneedle within transcanal endoscopic ear surgery (TEES).
Main Methods:
- Surgical procedures were developed and refined using seven cadaveric specimens.
- TEES involved tympanic cavity visualization, RWM exposure, and stapes footplate perforation for microinjection.
- Feasibility of different pipetting microneedles was evaluated for optimal trans-RWM delivery.
Main Results:
- TEES provided easy access to the RWM microinjection site.
- A soft-connected pipetting microneedle was identified as most suitable for trans-RWM microinjection.
- Visible fluid outflow confirmed successful inner ear drug delivery, validated in a clinical trial.
Conclusions:
- Trans-RWM microinjection using a soft-connected pipetting microneedle via TEES is a feasible approach for inner ear gene therapy delivery.
- This method offers a viable pathway for advancing clinical inner ear treatments.
