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Updated: Jun 8, 2025
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Monitoring Protein Adsorption with Solid-state Nanopores
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Improving macromolecule crowding configurations in nanopores for protein sensing
Fei Zheng1,2,3,4, HongLuan Li1,2, Jun Yang1,2
1Jiangsu Key Laboratory for Design and Manufacture of Micro-nano Biomedical Instruments, Southeast University, Nanjing 211189, China. major212@seu.edu.cn.
Chemical Communications (Cambridge, England)
|November 8, 2024
Abstract:
We show that PEG-induced macromolecular crowding enhances protein detection in nanopores by increasing capture rate and translocation frequency. Experimental data indicate that a PEG concentration gradient boosts capture efficiency, while our theoretical model attributes this enhancement to osmotic flow, offering insights for improving nanopore-based biosensing.
