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Related Experiment Video

Updated: Jun 17, 2026

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
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A Multi-Isotopic Chemometric Approach for Tracing Hazelnut Origins.

Berta Torres-Cobos1,2, Mònica Rosell3, Albert Soler3

  • 1Departament de Nutrició, Ciències de l'Alimentació i Gastronomia, Universitat de Barcelona, Av Prat de La Riba, 171, 08921 Santa Coloma de Gramenet, Spain.

Foods (Basel, Switzerland)
|November 9, 2024
PubMed
Summary

Authenticating hazelnut origin is crucial for preventing fraud. Stable isotope analysis, using oxygen-18 (δ18O), deuterium (δ2H), and carbon-13 (δ13C) in fatty acids, accurately identified hazelnut provenance.

Keywords:
PLS-DAfood fraudgeographical authenticationhazelnutstable isotopes

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Area of Science:

  • Food science
  • Analytical chemistry
  • Agricultural science

Background:

  • High-value products like hazelnuts face fraud risks due to origin-based pricing.
  • Ensuring hazelnut authenticity is vital for consumer trust and food safety.
  • Stable isotope analysis is a reliable method for food origin authentication.

Purpose of the Study:

  • To evaluate the effectiveness of bulk oxygen-18 (δ18O) and fatty acid deuterium (δ2H) and carbon-13 (δ13C) as markers for hazelnut origin authenticity.
  • To develop and validate chemometric models for differentiating hazelnut origins.

Main Methods:

  • Analysis of bulk δ18O values in hazelnuts.
  • Measurement of δ2H and δ13C in the main fatty acids of hazelnuts.
  • Development of Partial Least Squares Discriminant Analysis (PLS-DA) models for classification.

Main Results:

  • A multi-isotopic approach combining δ18O, δ2H, and δ13C showed significant potential for origin authentication.
  • PLS-DA models achieved a 94% global correct classification rate in discriminating hazelnuts from different geographical regions.
  • The study demonstrated the feasibility of using these isotopic markers for robust hazelnut provenance determination.

Conclusions:

  • The combined use of bulk δ18O and fatty acid isotopes (δ2H, δ13C) is a promising strategy for authenticating hazelnut origin.
  • This multi-isotopic approach offers a reliable method for combating food fraud in high-value products.
  • Further research should expand the model to include more production areas and harvest years for broader applicability.