Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimized chemotherapy sequence for metastatic pancreatic ductal adenocarcinoma patients: FOLFIRINOX followed by Gemcitabine plus nab-paclitaxel.

BMC cancer·2026
Same author

Downregulation of the Long Non-Coding RNA <i>MDL1AS</i> Alters Metabolism, Differentiation, and Radiosensitivity in NTERA2 and SH-SY5Y Cells.

Cancers·2026
Same author

A Stable RNA Vaccine Against the Regulatory Peptide Adrenomedullin Reduces Angiogenesis and Tumor Burden in a Subcutaneous Melanoma Model Without Inducing an Immunosuppressive Tumor Microenvironment.

International journal of molecular sciences·2025
Same author

Cripto-1 and Cripto-3 differential expression pattern in human placenta, umbilical cord and fetal tissues suggests new functions for these proteins.

Scientific reports·2025
Same author

Targeting Casein Kinase 2 and Histone Deacetylase with a Dual Inhibitor Effectively Reduces Tumor Growth in a Triple-Negative Breast Cancer Xenograft Model.

ACS pharmacology & translational science·2025
Same author

Neuroprotective Effect of Methylene Blue in a Rat Model of Traumatic Optic Neuropathy.

Pharmaceuticals (Basel, Switzerland)·2025

Related Experiment Video

Updated: Jun 7, 2025

Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells
08:33

Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells

Published on: April 25, 2014

19.8K

Human Cripto-1 and Cripto-3 Protein Expression in Normal and Malignant Settings That Conflicts with Established

Frank Cuttitta1, Josune García-Sanmartín2, Yang Feng1

  • 1Tumor Angiogenesis Unit, Mouse Cancer and Genetics Program, National Cancer Institute/Frederick Facility, Frederick, MD 21701, USA.

Cancers
|November 9, 2024
PubMed
Summary

New antibodies distinguish Cripto-1 (CR1) and Cripto-3 (CR3) proteins, confirming CR3 is translated in tumors and sera. This clarifies CR1/CR3 roles in cancer and cell signaling.

Keywords:
Cripto-1Cripto-3carcinogenesiscell signal transductiondiscriminationmonoclonal antibodyoncofetal proteinpseudogene

More Related Videos

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

8.0K
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

10.8K

Related Experiment Videos

Last Updated: Jun 7, 2025

Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells
08:33

Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells

Published on: April 25, 2014

19.8K
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

8.0K
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

10.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Cripto-1 (CR1) is an embryonic protein vital for implantation, re-expressed in adults during repair, inflammation, and cancer.
  • CR1 and its pseudogene product Cripto-3 (CR3) are highly homologous, making discrimination difficult with existing antibodies.

Purpose of the Study:

  • To develop specific monoclonal antibodies (MoAbs) capable of differentiating between human CR1 and CR3 proteins.
  • To investigate the expression and function of CR3 in human cancer and its interaction with other signaling molecules.

Main Methods:

  • Generation of high-affinity mouse monoclonal antibodies (MoAbs) with specific binding to human CR1/CR3.
  • Validation of antibody specificity and lack of cross-reactivity.
  • Detection of CR3 protein in tumor tissues, cell lysates, and patient sera.
  • Analysis of CR1 and CR3 competition for binding to Nodal, GRP78, and Alk4.

Main Results:

  • Developed discriminatory MoAbs confirming CR3 as a translated protein in human tumors, cancer cell lysates, and patient sera.
  • Demonstrated that CR1 and CR3 compete for binding to Nodal, GRP78, and Alk4.
  • Challenged existing conventions regarding CR1/CR3 protein expression and clarified their roles.

Conclusions:

  • CR3 is a bona fide protein involved in human carcinogenesis and cell signaling pathways.
  • The new MoAbs are valuable reagents for resolving CR1/CR3 expression ambiguities.
  • Findings have potential clinical relevance for cancer patient prognosis and disease severity assessment.