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A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment
Published on: May 24, 2022
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Label-Free Detection of Breast Phyllodes Tumors Based on Multiphoton Microscopy.
Xi Chen1, Junzhen Jiang1, Liwen Hu2
1Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou, China.
Journal of Biophotonics
|November 9, 2024
Summary
Multiphoton microscopy (MPM) can detect phyllodes tumors (PTs) in breast tissue without labels. Changes in collagen fiber structure indicate PT malignancy, aiding diagnosis and grading.
Area of Science:
- Biomedical Optics
- Pathology
- Medical Imaging
Background:
- Phyllodes tumors (PTs) are rare breast neoplasms requiring accurate diagnosis for treatment.
- Distinguishing PTs from normal tissue and grading malignancy is clinically significant.
Purpose of the Study:
- To evaluate multiphoton microscopy (MPM) for label-free detection and differentiation of PTs.
- To establish optical diagnostic characteristics of PTs based on stromal collagen morphology.
Main Methods:
- Utilized multiphoton microscopy (MPM) with two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) imaging.
- Developed an automated image processing strategy to quantify collagen fiber morphology in PT stroma and boundaries.
Main Results:
- MPM successfully detected and differentiated PTs from normal breast tissue.
- Morphological alterations in collagen fibers were identified as key indicators for PT diagnosis and grading (benign, borderline, malignant).
Conclusions:
- MPM offers a label-free approach for PT detection and grading.
- Collagen fiber analysis using MPM provides new insights for diagnosing and staging phyllodes tumors.
Keywords:
collagen fiber characteristicsmultiphoton microscopyphyllodes tumorssecond‐harmonic generation
