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Updated: Jun 7, 2025

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
Exploring the influence of moisture stress on microbial-driven organic acid synthesis in potato waste fermentation
Jiawei Li1, Xiaofeng Guan2, Wenming Huang3
1College of Animal Science and Technology, Southwest University, Chongqing, PR China; National Center for Technology Innovation for Pigs, Chongqing, PR China.
Abstract:
Anaerobic fermentation of potato leaves and stems for organic acid synthesis, serving as food additives, faces impediments due to misconceptions about the effects of moisture stress on the acid-synthesizing microbiome. An ingenious method, avoiding interference from microbiome and nutrient integrations, was employed in the present study. Results showed that increasing the moisture level from 60 % to 75 % significantly improved lactic acid (182.64 %), acetic acid (163.55 %), propionic acid (1960.43 %), nonprotein nitrogen, free amino acid and ammonia levels but reduced pH value and water-soluble carbohydrate and hemicellulose levels. Microbiologically, the high-moisture groups enriched Lactiplantibacillus, Levilactobacillus and Enterobacter, upregulated glycolysis, nitrogen, pyruvate and propanoate metabolisms, and activated genes for acid-producing and ammonia-forming enzymes. Notably, Lactiplantibacillus and Enterobacter prevailed in glycolysis and nitrogen metabolism, respectively, and Levilactobacillus was more prominent in pyruvate and propanoate metabolism under high-moisture conditions. Collectively, a moisture level of 75 % benefited organic acid synthesis from potato waste via anaerobic fermentation.
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