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Updated: Jun 7, 2025
Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
Published on: July 7, 2020
PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing
Adam M Blanchard1, Adam Taylor2, Andrew Warry2
1University of Nottingham, School of Veterinary Medicine and Science, Leicestershire LE12 5RD, United Kingdom.
Motivation:
Current methods for visualising and interrogating high-throughput transposon insertion mutagenesis sequencing (TIS) data requires a significant time investment in learning bioinformatics, often producing static figures that do not facilitate real time analysis.
Summary:
We have created an accessible web-based browser tool for visualisation and downstream analysis of high-throughput TIS data results generated by the PIMMS analysis pipeline. This includes multiple interactive and sortable tables to aid the user to identify genes of interest, enabling the user to gain a greater understanding of the genes contributing to fitness in their experimental work. PIMMS-Dash permits researchers, with any level of bioinformatics knowledge, to interrogate data sets and generate publication quality figures.
Availability:
PIMMS-Dash is freely available and is accessible online at https://pimms-dashboard-uon.azurewebsites.net and a Docker containerised version is available at https://github.com/Streptococcal-Research-Group/PIMMS-Dash to run locally.
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