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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
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Current status and trends in forest genomics
Dulal Borthakur1, Victor Busov2, Xuan Hieu Cao3
1Dulal Borthakur, Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, 1955 East-West Road, Honolulu, HI 96822, USA.
Forestry Research
|November 11, 2024
Summary
Forest genomics research is rapidly advancing, utilizing new technologies to improve forest productivity and climate change adaptation. This review covers 12 branches, highlighting future directions for integrative genomics in forest biology.
Area of Science:
- Forestry
- Genomics
- Plant Biotechnology
Background:
- Forest ecosystems are vital but threatened by climate change, deforestation, and degradation.
- Forest genomics offers solutions for enhancing forest productivity and climate resilience.
Purpose of the Study:
- To review the advancement and current status of 12 research branches in forest genomics.
- To provide future research directions and focuses for each area.
Main Methods:
- High-throughput sequencing technologies
- Single-cell RNA sequencing (scRNA-seq)
- CRISPR-mediated genome editing
- Spatial transcriptomics
- Bioinformatics analysis
- Plant biotechnology
Main Results:
- Rapid advancements in forest genomics over the last two decades.
- Generation of multidimensional, multilayered, and spatiotemporal gene expression data.
- Enabling elucidation of molecular mechanisms underlying phenotypic variation.
Conclusions:
- A shift towards advanced, integrative genomics research in forestry is emerging.
- New setups are being established for investigating spatiotemporal development and differentiation in forest genomics.
Keywords:
CRISPR-mediated genome editingForest genetic diversity and climate adaptionGenome assemblyHaploid inductionPerennial growth and seasonality regulationQTL, association Studies, and genomic selectionSingle cell RNA-seqSystems biology and data analysisTransformation and regenerationWood formation
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