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Related Experiment Video

Updated: Jun 24, 2026

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

Published on: January 26, 2017

Genome-Wide Profiling of Histone Modifications in Fission Yeast Using CUT&Tag.

Sito Torres-Garcia^1,2, Yiwen Huang^1,3, Felix Selasi Dewornu¹

¹Wellcome Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 11, 2024

Summary

This summary is machine-generated.

We adapted the CUT&Tag technique for epigenomic profiling in fission yeast. This method requires less input material and offers a streamlined protocol for studying histone modifications.

Keywords:

CUT&Tag ChIP-Seq Epigenome Heterochromatin Histone modifications Nucleosome Schizosaccharomyces pombe

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Area of Science:

Molecular Biology
Genetics
Epigenetics

Background:

Eukaryotic DNA is organized into chromatin, with nucleosomes as the basic unit.
Histone posttranslational modifications (PTMs) and variants regulate genome structure and activity.
Genome-wide profiling is crucial for understanding genome function.

Purpose of the Study:

To adapt the Cleavage Under Targets and Tagmentation (CUT&Tag) method for epigenomic profiling in fission yeast (Schizosaccharomyces pombe).
To establish a streamlined and low-input protocol for analyzing epigenetic marks in this organism.

Main Methods:

Adaptation of the CUT&Tag protocol for fission yeast.
Benchmarking the adapted protocol using the heterochromatin-associated histone H3K9 methylation mark.
Utilizing next-generation sequencing for genome-wide profiling.

Main Results:

Successful adaptation of the CUT&Tag protocol for fission yeast.
Demonstration of the protocol's efficiency in profiling histone modifications.
The adapted method requires significantly less input chromatin and antibodies compared to traditional ChIP-Seq.

Conclusions:

The adapted CUT&Tag protocol provides an efficient and accessible method for epigenomic profiling in fission yeast.
This advancement facilitates the study of genome regulation and function in Schizosaccharomyces pombe.
The protocol's low-input requirement and streamlined nature make it valuable for various epigenomic studies.