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Related Experiment Videos

Plasma separation using a hollow fiber membrane device.

D H Buchholz, A Lin, E Snyder

    Transfusion
    |March 1, 1986
    PubMed
    Summary

    A novel hollow fiber device efficiently separates plasma from blood during therapeutic plasma exchange. This safe and effective device shows no complement activation, making it suitable for clinical use.

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    Area of Science:

    • Biomedical Engineering
    • Hematology
    • Immunology

    Background:

    • Therapeutic plasma exchange (TPE) is a critical procedure for removing harmful substances from blood.
    • Efficient and safe devices are needed to improve TPE efficacy and patient outcomes.

    Purpose of the Study:

    • To evaluate a new disposable hollow fiber device for plasma separation during TPE.
    • To assess the device's efficiency, safety, and impact on complement pathways.

    Main Methods:

    • Collected plasma from normal donors (n=43) and performed sham (n=10) and therapeutic (n=12) plasma exchanges.
    • Processed blood at 70 ml/min, measuring plasma flux and separation efficiencies.
    • Assessed cell contamination, plasma hemoglobin, and complement activation (CH50, AP50, C3, C5).

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  • Determined sieving coefficients for various plasma proteins and factors.

    • Main Results:

    • The device achieved plasma flux of 23-25 ml/min with separation efficiencies of 52-60%.
    • Procedures were well-tolerated with negligible cell contamination and low hemoglobin levels in separated plasma.
    • No evidence of in vivo complement activation (classical or alternative pathways) was observed.
    • Sieving coefficients indicated efficient separation of plasma components, with values near 1.0 for most proteins and factors.

    Conclusions:

    • The disposable hollow fiber device effectively separates cell-free plasma from blood.
    • The device demonstrates safety and efficacy for use in therapeutic plasma exchange procedures.
    • It shows potential for clinical application in TPE, with no adverse effects on complement pathways.