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An evaluation of 11 red cell elution procedures
Eleven elution procedures were evaluated for their efficacy in recovering antibody from red cells sensitized with immunoglobulin. The procedures evaluated included eight published methods (Lui, heat, digitonin-acid, ether, chloroform, dichloromethane, xylene, and alcohol freeze-thaw) and three commercial elution kits (EluAid, Ortho Diagnostic Systems, Inc.; Elution Solution, Biological Corporation of America; and Elu-Kit II, Gamma Biologicals, Inc.). The xylene elution method proved to be the most effective method, followed by Elu-Kit II, chloroform, dichloromethane, and digitonin-acid. The other six methods evaluated were not optimal based on the suitability of each procedure and the calculated sensitivity.
Eleven elution procedures were evaluated for their efficacy in recovering antibody from red cells sensitized with immunoglobulin. The procedures evaluated included eight published methods (Lui, heat, digitonin-acid, ether, chloroform, dichloromethane, xylene, and alcohol freeze-thaw) and three commercial elution kits (EluAid, Ortho Diagnostic Systems, Inc.; Elution Solution, Biological Corporation of America; and Elu-Kit II, Gamma Biologicals, Inc.). The xylene elution method proved to be the most effective method, followed by Elu-Kit II, chloroform, dichloromethane, and digitonin-acid. The other six methods evaluated were not optimal based on the suitability of each procedure and the calculated sensitivity.
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