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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

311
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
311
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

296
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
296

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Surface-Enhanced Raman Spectroscopy: A Game Changer for Metabolomics Research.

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Surface-Enhanced Raman Scattering-Based Multimodal Techniques: Advances and Perspectives.

Emily Xi Tan1, Qi-Zhi Zhong1, Jaslyn Ru Ting Chen1

  • 1School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, 21 Nanyang Link, 637371 Singapore.

ACS Nano
|November 12, 2024
PubMed
Summary

Surface-enhanced Raman scattering (SERS) spectroscopy offers versatile molecular fingerprinting. Emerging multimodal SERS techniques enhance sensitivity and reliability for complex biochemical detection and bioimaging.

Keywords:
SERSbioimagingchemical analysismachine learningmultimodal detectionsurface-enhanced Raman scatteringtheragnosistheragnostic

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biotechnology

Background:

  • Surface-enhanced Raman scattering (SERS) is a powerful molecular fingerprinting technique known for its speed, aqueous compatibility, and portability.
  • Real-world SERS applications face challenges like sample variability, matrix effects, and nonlinear responses in complex biological and chemical environments.
  • Overcoming these limitations is crucial for advancing SERS in detection, bioimaging, and theranostics.

Purpose of the Study:

  • To highlight emerging SERS-based multimodal techniques.
  • To address key roadblocks in SERS sensitivity, specificity, and reliability.
  • To discuss advancements for improved biochemical detection, bioimaging, and theranostics.

Main Methods:

  • Categorization of SERS-based multimodal techniques into complementary and orthogonal approaches.
  • Discussion of how these methods compensate for individual weaknesses or provide corroborating results.
  • Exploration of multimodal techniques for analyzing a broader range of analytes, from small molecules to tissues.

Main Results:

  • Multimodal SERS approaches enhance information gain from single experiments.
  • These techniques improve qualitative and quantitative analysis of analytes.
  • The scope of detectable analytes is broadened, including complex biological samples.

Conclusions:

  • SERS-based multimodal techniques are essential for overcoming current analytical limitations.
  • Future directions involve multimodal platform design, instrument integration, and advanced data analytics.
  • These advancements promise to push the analytical boundaries of holistic detection.