Related Experiment Video
Updated: Jun 9, 2026

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
Published on: January 9, 2020
Surface-Enhanced Raman Spectroscopy: A Game Changer for Metabolomics Research
Xinyuan Bi1, Xing Yi Ling2,3, Jian Ye1,4,5,6
1School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
None:
Metabolomic detection enables a systems-level understanding of biological processes, while many emerging demands remain unmet. Surface-enhanced Raman spectroscopy (SERS) has recently evolved into a promising platform for metabolic detection yet not reaching true metabolomics. This Mini-Review is motivated by recent advances in understanding molecule-nanomaterial interactions aimed at addressing the related limitations. We first outline the fundamental principles enabling SERS-based metabolomic detection, including specificity, sensitivity, near-field compatibility with small metabolites, and nondestructiveness. Gaps between current SERS techniques and true metabolomics are delineated, and the key technical advances to overcome these challenges are also highlighted, including digital SERS, SERSome, molecule-resolvable SERSome, probe-functionalized nanomaterials, and artificial intelligence-assisted analysis. These developments have enabled SERS across multiple analytical paradigms, spanning targeted detection, phenotypic profiling, and emerging metabolomics. At last, we discuss the future challenges in hopes of advancing SERS from a sensing-oriented technique to a true metabolomic platform, ultimately facilitating the decoding of biological systems.
Related Concept Videos
MALDI-TOF Mass Spectrometry
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...