A Perspective of Multi-Reflecting TOF MS.
A N Verenchikov1, V V Makarov1, A V Vorobyev1
1Mass Spectrometry Consulting Ltd, City of Bar, Montenegro.
Mass Spectrometry Reviews
|November 12, 2024
Summary
Multi-pass time-of-flight mass spectrometry (TOF MS) analyzers, specifically multi-reflecting (MRT) designs, offer enhanced resolution for complex omics studies. This review details their two-decade evolution and practical applications.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Omics Technologies
Background:
- Time-of-flight mass spectrometry (TOF MS) is crucial for rapid, high-sensitivity analysis.
- Increasing sample complexity in omics necessitates higher resolving power in TOF MS.
- Traditional TOF instruments require large sizes to achieve high resolution.
Purpose of the Study:
- To review two decades of advancements in multi-pass time-of-flight (MPT) analyzers.
- To highlight the evolution and benefits of multi-reflecting (MRT) TOF MS instruments.
- To provide researchers with information for selecting appropriate MRT mass spectrometers.
Main Methods:
- Review of multi-pass TOF analyzer designs, focusing on multi-turn (MTT) and multi-reflecting (MRT) configurations.
- Analysis of optimized analyzer designs, including mirror-based MRT instruments.
- Exploration of orthogonal accelerators and trap pulse converters, assessing efficiency and space charge effects.
Main Results:
- MPT analyzers, particularly MRT designs, overcome the size limitations of traditional TOF MS for high resolution.
- Mirror-based MRT instruments generally offer superior resolution and spatial acceptance compared to MTT designs.
- Comparison of various MRT configurations and commercial instruments is presented.
Conclusions:
- Multi-reflecting (MRT) TOF MS technology represents a significant advancement for high-resolution analysis in complex omics studies.
- Optimized analyzer designs and components like orthogonal accelerators are key to performance.
- This review empowers researchers to make informed decisions regarding MRT mass spectrometer selection.
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