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A Novel Compact Multi-Reflecting Time-of-Flight Mass Spectrometer.

Anatoly N Verenchikov1, Jason Wildgoose2, Sergey N Kirillov1

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Journal of the American Society for Mass Spectrometry
|January 26, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a novel liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) instrument for rapid, accurate analysis of complex biological mixtures like lipids. Its balanced speed, resolution, and dynamic range offer enhanced performance for metabolomics and proteomics.

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high resolutionlipidomicsmultireflecting time-of-flight

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Mass spectrometry is crucial for analyzing complex biological mixtures in fields like metabolomics, lipidomics, and proteomics.
  • High-speed instruments with sub-ppm mass accuracy and wide dynamic range are essential for these applications.

Purpose of the Study:

  • To introduce a novel liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) quadrupole time-of-flight mass spectrometer.
  • To highlight its innovative features: a new collision cell, high dynamic range detector, and compact multireflecting orthogonal time-of-flight analyzer.

Main Methods:

  • Development and characterization of a new LC-MS/MS quadrupole time-of-flight mass spectrometer.
  • Evaluation of instrument performance including resolution, acquisition speed, dynamic range, and linearity.
  • Analysis of isotopically labeled lipids in human blood plasma to demonstrate capabilities.

Main Results:

  • The instrument achieves 100,000 FWHM resolution at 100 spectra/s acquisition speed.
  • Demonstrates excellent linearity (R 2 = 0.984) within a 105 dynamic range.
  • Successfully analyzed isotopically labeled lipids in human blood plasma, showcasing balanced performance.

Conclusions:

  • The novel LC-MS/MS instrument offers a balanced combination of speed, resolution, and dynamic range.
  • This performance makes it suitable for demanding applications in metabolomics, lipidomics, and proteomics.
  • The instrument represents a significant advancement in mass spectrometry for complex mixture analysis.