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NAD(P)-Dependent Glucose Dehydrogenases: Underestimated Multifunctional Biocatalysts
Rohit Kumar1, Samantha J Tambrini1, Guangde Jiang1
1Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI, 48201, USA.
Chembiochem : a European Journal of Chemical Biology
|November 12, 2024
Summary
NAD(P)-dependent glucose dehydrogenases (GDHs) are versatile biocatalysts beyond cofactor regeneration. These enzymes show significant potential in synthesizing drug molecules and intermediates through various chemical transformations.
Area of Science:
- Biocatalysis
- Enzyme Engineering
- Organic Synthesis
Background:
- NAD(P)-dependent glucose dehydrogenases (GDHs) are crucial enzymes in biocatalysis.
- Traditionally, GDHs are used for NAD(P)H regeneration in enzymatic reactions.
- Recent research reveals broader applications for GDHs in chemical synthesis.
Purpose of the Study:
- To review recent advancements in the multifunctional roles of GDHs in biocatalysis.
- To highlight the expanding applications of GDHs in small molecule synthesis.
- To emphasize the significant potential of GDHs as biocatalysts.
Main Methods:
- Literature review of recent studies on GDHs.
- Analysis of GDH-catalyzed reactions in small molecule synthesis.
- Focus on advancements in understanding GDH versatility.
Main Results:
- GDHs catalyze diverse reactions including ketone reduction, imine reduction, and aldehyde reduction.
- GDHs are effective in synthesizing drug molecules and intermediates.
- The scope of GDHs extends beyond cofactor regeneration.
Conclusions:
- GDHs possess significant potential as versatile biocatalysts in chemical transformations.
- Advancements in understanding GDHs unlock new applications in synthesis.
- GDHs are key enzymes for future biocatalytic applications in drug discovery and development.
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