Related Experiment Video

Updated: Jun 7, 2025

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells
09:01

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

Published on: March 7, 2025

296

Comprehensive analysis of the relationship between RNA modification writers and immune microenvironment in head and

Wei Li1, Ying Chen2, Yao Zhang3

  • 1The First Clinical College of China Medical University, Shenyang, China.

BMC Immunology
|November 12, 2024
PubMed
Abstract

Insights

This study identified two RNA modification clusters in head and neck squamous cell carcinoma (HNSCC) that correlate with the tumor immune microenvironment, offering a basis for precision immunotherapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Head and neck squamous cell carcinoma (HNSCC) is a significant global health concern.
  • RNA modification writers (m6A, m1A, A-I editing, APA) play a role in cancer development and the tumor microenvironment (TME).

Purpose of the Study:

  • To comprehensively investigate the role of four RNA modification writers in HNSCC progression.
  • To explore the relationship between RNA modification patterns and the HNSCC immune microenvironment.

Main Methods:

  • Utilized The Cancer Genome Atlas (TCGA) database for transcription profile data.
  • Clustered HNSCC patients based on RNA modification writers and developed a Writer-score system.
  • Validated the Writer-score system and analyzed its association with immunological characteristics.

Main Results:

  • Identified two distinct RNA modification clusters (A and B) in HNSCC.
  • Demonstrated a strong association between these RNA modification clusters (Writer-score) and key immunological features.
  • These features include immunomodulators, cancer immunity cycles, infiltrating immune cells, inhibitory immune checkpoints, and T cell inflamed scores.

Conclusions:

  • The study identified two novel RNA modification clusters in HNSCC.
  • These clusters show a significant relationship with the tumor's immunological characteristics.
  • Findings provide a new theoretical foundation for developing precision immunotherapy strategies for HNSCC patients.
Keywords:
HNSCCImmunotherapyRNA modification writersTumor microenvironmentUnsupervised clustering

More Related Videos

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples07:43

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Published on: July 29, 2017

9.6K
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma10:26

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Published on: March 11, 2014

27.3K

Related Concept Videos

Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.7K
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.9K
Histone Modification02:32

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.1K