Velocity coding in the central brain of bumblebees

Moving animals experience wide-field optic flow due to the displacement of the retinal image during motion. These cues provide information about self-motion and are important for flight control and stabilization, and for more complex tasks like path integration. Although in honeybees and bumblebees the use of wide-field optic flow in behavioral tasks is well investigated, little is known about the underlying neuronal processing of these cues. Furthermore, there is a discrepancy between the temporal frequency tuning observed in most motion-sensitive neurons described so far from the optic lobe of insects and the velocity tuning that has been shown for many behaviors. Here, we investigated response properties of motion-sensitive neurons in the central brain of bumblebees. Extracellular recordings allowed us to present a large number of stimuli to probe the spatiotemporal tuning of these neurons. We presented moving gratings that simulated either front-to-back or back-to-front optic flow and found three response types. Direction-selective responses of one of the groups matched those of TN-neurons, which provide optic flow information to the central complex, whereas the other groups contained neurons with purely excitatory responses that were either selective or nonselective for stimulus direction. Most recorded units showed velocity-coding properties at lower angular velocities, but showed spatial frequency-dependent responses at higher velocities. Based on behavioral data, neuronal modeling work has previously predicted the existence of nondirection-selective neurons with such properties. Our data now provide physiological evidence for these neurons and show that neurons with TN-like properties exhibit a similar velocity-dependent coding.NEW & NOTEWORTHY Using extracellular recordings, we show that neurons in the central brain and central complex of bumblebees show velocity coding properties.