Visualization of Endogenous RNA G-Quadruplex in Individual Genes in Single Cells

Single-cell RNA imaging enables the detection of endogenous RNA and the analysis of its abundance, localization, and single-cell heterogeneity, which is powerful in the study of RNA biology. G-quadruplex (G4) is a kind of nucleic secondary structure and plays important roles in various biological processes. To reveal the regulating mechanism of monogenic G4s in a specific biological pathway, it is of great important to visualize endogenous RNA G4 in individual genes. Here, we describe Module Assembled Multifunctional Probes Assay (MAMPA) for single-cell visualization of monogenic RNA G4. This method works well in various cell lines and various RNA G4 analytes and has a low detection limit at ten copies per cell.