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Updated: Jun 7, 2025
Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
Published on: September 18, 2014
Intracellularly synthesized ssDNA for continuous genome engineering.
Wenqian Liu1, Yingjia Pan2, Yu Zhang1
1Key Laboratory of Biomass Chemical Engineering of Ministry of Education and State Key Laboratory of Biobased Transportation Fuel Technology, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China; BGI Research, Hangzhou 310030, China.
Single-stranded DNA (ssDNA)-mediated genome editing offers a promising solution for dynamic, continuous gene mutation. This review covers intracellular ssDNA production systems and their applications in genome engineering and molecular recording.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Current genome editing tools face limitations in dynamic and continuous applications.
- In vivo single-stranded DNA (ssDNA)-mediated mutation presents a novel approach for continuous genome editing.
Purpose of the Study:
- To review intracellular ssDNA production systems for genome engineering.
- To explore applications of retron-based systems in molecular recording and gene editing.
- To discuss challenges and future prospects of ssDNA-mediated continuous genome editing.
Main Methods:
- Literature review of intracellular ssDNA production systems.
- Analysis of genome engineering achievements in prokaryotic and eukaryotic cells.
- Review of retron-based system applications.
Main Results:
- Various intracellular ssDNA production systems have been developed.
- Significant achievements in genome engineering using ssDNA have been reported.
- Retron-based systems show potential in molecular recording, multiplex editing, and functional screening.
Conclusions:
- ssDNA-mediated genome editing is a powerful tool for continuous genetic modification.
- Retron-based systems offer diverse applications beyond basic genome editing.
- Further research is needed to overcome current challenges and unlock the full potential of ssDNA-mediated editing.
