Related Experiment Video

Updated: Jul 31, 2026

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
14:27

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity

Published on: August 19, 2013

Determination of Michaelis-Menten parameters from initial velocity measurements using unstable substrate

T Keleti

    Analytical Biochemistry
    |January 1, 1986
    PubMed

    More Related Videos

    Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions13:00

    Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

    Published on: April 4, 2014

    Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes09:42

    Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

    Published on: January 16, 2016

    Related Concept Videos

    Enzyme Kinetics01:19

    Enzyme Kinetics

    Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
    Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
    Measuring Reaction Rates03:09

    Measuring Reaction Rates

    Polarimetry finds application in chemical kinetics to measure the concentration and reaction kinetics of optically active substances during a chemical reaction. Optically active substances have the capability of rotating the plane of polarization of linearly polarized light passing through them—a feature called optical rotation. Optical activity is attributed to the molecular structure of substances. Normal monochromatic light is unpolarized and possesses oscillations of the electrical field in...
    Arrhenius Plots02:34

    Arrhenius Plots

    The Arrhenius equation relates the activation energy and the rate constant, k, for chemical reactions. In the Arrhenius equation, k = Ae−Ea/RT, R is the ideal gas constant, which has a value of 8.314 J/mol·K, T is the temperature on the kelvin scale, Ea is the activation energy in J/mole, e is the constant 2.7183, and A is a constant called the frequency factor, which is related to the frequency of collisions and the orientation of the reacting molecules.
    The Arrhenius equation can be used to...
    Introduction to Enzyme Kinetics01:19

    Introduction to Enzyme Kinetics

    Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
    The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
    Catalytically Perfect Enzymes01:07

    Catalytically Perfect Enzymes

    The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
    Turnover Number and Catalytic Efficiency01:19

    Turnover Number and Catalytic Efficiency

    The turnover number of an enzyme is the maximum number of substrate molecules it can transform per unit time. Turnover numbers for most enzymes range from 1 to 1000 molecules per second. Catalase has the known highest turnover number, capable of converting up to 2.8×106 molecules of hydrogen peroxide into water and oxygen per second. Lysozyme has the lowest known turnover number of half a molecule per second.
    Chymotrypsin is a pancreatic enzyme that breaks down proteins during digestion. The...