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Updated: Jun 7, 2025

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species
Published on: March 29, 2019
SPL13 controls a root apical meristem phase change by triggering oriented cell divisions
Baojun Yang1,2,3,4, Yanbiao Sun1,2, Max Minne1,2
1Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Abstract:
Oriented cell divisions are crucial for determining the overall morphology and size of plants, but what controls the onset and duration of this process remains largely unknown. Here, we identified a small molecule that activates root apical meristem (RAM) expression of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE13 (SPL13) a known player in the shoot's juvenile-to-adult transition. This expression leads to oriented cell divisions in the RAM through SHORT ROOT (SHR) and cell cycle regulators. We further show that the RAM has distinct juvenile and adult phases typed by morphological and molecular characteristics and that SPL factors are crucially required for this transition in Arabidopsis and rice (Oryza sativa). In summary, we provide molecular insights into the age-dependent morphological changes occurring in the RAM during phase change.
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