Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<b>Three new species of <i>Azana</i> Walker (Diptera: Mycetophilidae: Sciophilinae) from South Korea</b>.

Zootaxa·2026

Same author

Genome-wide association study on soybean canopy wilting under drought stress conditions in a rainout-shelter greenhouse.

Frontiers in plant science·2026

Same author

Quantitative trait loci analysis identifying novel genomic regions for off-target dicamba tolerance in soybean [Glycine max (L.) Merr.].

BMC plant biology·2026

Same author

Designable van der Waals Crystal for Artificial Neuronal Cell Mimicking.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

New Measurement Method for Lateral Acromial Protrusion as an Alternative to Critical Shoulder Angle, Independent of Sagittal Glenoid Tilt.

Clinics in orthopedic surgery·2026

Same author

High Polarity Doping of CoFe Layered Hydroxides: Bifunctional and Corrosion-Resistant Anion Exchange Membrane Seawater Electrolyzers.

Nano-micro letters·2026

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering

Published on: February 12, 2013

High-Resolution Total Internal Reflection-Based Structural Coloration by Electrohydrodynamic Jet Printing of

Dongho Lee¹, Doyoung Byun^1,2, Dae-Hyun Cho^3,4

¹Department of Mechanical Engineering, Sungkyunkwan University, 2066 Seobu-Ro, Jangan-Gu, Suwon-Si 16419, Republic of Korea.

ACS Macro Letters

|November 14, 2024

Summary

Electrohydrodynamic (EHD) jet printing enables high-resolution, full-color structural coloration using total internal reflection (TIR) microdomes. This technique offers advanced anticounterfeiting solutions with precise control over microdome size.

More Related Videos

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

High-resolution Patterning Using Two Modes of Electrohydrodynamic Jet: Drop on Demand and Near-field Electrospinning

High-resolution Patterning Using Two Modes of Electrohydrodynamic Jet: Drop on Demand and Near-field Electrospinning

Published on: July 10, 2018

Related Experiment Videos

Last Updated: Jun 13, 2026

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering

Density Gradient Multilayered Polymerization DGMP: A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering

Published on: February 12, 2013

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

High-resolution Patterning Using Two Modes of Electrohydrodynamic Jet: Drop on Demand and Near-field Electrospinning

High-resolution Patterning Using Two Modes of Electrohydrodynamic Jet: Drop on Demand and Near-field Electrospinning

Published on: July 10, 2018

Area of Science:

Optics and Photonics
Materials Science
Nanotechnology

Background:

Total internal reflection (TIR) structural coloration offers an alternative to traditional nanostructure-based color generation.
Existing solution-based printing methods struggle with achieving full-color capabilities due to resolution limits.

Purpose of the Study:

To develop a high-resolution, full-color structural coloration method using TIR.
To explore the application of this technique in anticounterfeiting measures.

Main Methods:

Utilized electrohydrodynamic (EHD) jet printing to fabricate transparent microdome structures with diameters ranging from 1.2 to 9.9 μm.
Investigated the relationship between microdome size and the resulting color through varying reflection paths.

Main Results:

Achieved unprecedented 3628 dpi resolution for full-color structural images.
Demonstrated precise control over microdome size, enabling tunable color properties.
Showcased the potential for encoding complex information within the structural color images.

Conclusions:

EHD jet printing provides a simple yet precise method for creating high-resolution TIR-based structural colors.
The developed technique is highly suitable for advanced anticounterfeiting applications due to its high resolution and information encoding capabilities.