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Updated: May 6, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Innumerable Culture-Negative Intracranial Abscesses in an Immunocompetent Young Male Diagnosed by 16s rRNA Gene
Ronald A Miller1, Yeonsoo Kim2, Vasyl Pryshliak3
1Neurology, State University of New York (SUNY) Upstate Medical University, Syracuse, USA.
Abstract:
Here, we present a case of a healthy young man with an initial presentation concerning for infectious encephalitis later found on neuroimaging to have extensive intracranial lesions. Pathological analysis of these lesions was consistent with a bacterial intraparenchymal abscess; however, cultures were negative for a causative organism. Results of gene sequencing from an abscess sample were consistent with Streptococcus intermedius infection. Streptococcus intermedius, a β-hemolytic gram-positive species of the Streptococcus anginosus group (SAG), is known to possess several virulence factors that promote abscess formation.
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