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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.1K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Genomics02:02

Genomics

36.0K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.0K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

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Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
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Updated: Jun 7, 2025

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

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The Evolution of Next-Generation Sequencing Technologies.

Olaitan Akintunde1, Trichina Tucker1, Valerie J Carabetta2

  • 1Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2024
PubMed
Summary
This summary is machine-generated.

Next-generation sequencing technologies have revolutionized DNA analysis, enabling rapid and extensive genetic information retrieval. These advancements significantly impact biomedical research, healthcare, and the global economy.

Keywords:
DNA-seqHigh-throughput sequencingNext-generation sequencingRNA-seqSingle-molecule sequencing

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • DNA encodes the genetic information for all life forms.
  • The discovery of DNA's double helix structure spurred interest in sequencing.
  • Advancements in sequencing technologies have accelerated research and applications.

Purpose of the Study:

  • To review the historical development of DNA sequencing technologies.
  • To examine current next-generation sequencing platforms.
  • To explore the applications of sequencing in biomedical research and beyond.

Main Methods:

  • Tracing the evolution of DNA sequencing techniques from early methods to automation.
  • Discussing improvements like fluorescent dyes and polymerase chain reaction (PCR).
  • Analyzing the capabilities of current next-generation sequencing platforms.

Main Results:

  • Sequencing capabilities have progressed from hundreds of base pairs in days to thousands in hours.
  • High-throughput sequencing technologies have broad applications in research, biotech, and healthcare.
  • Significant progress has been made, with ongoing potential for further improvements.

Conclusions:

  • Next-generation sequencing platforms represent a major leap in genetic analysis.
  • These technologies have a profound positive impact on human health and the economy.
  • Continued innovation in sequencing promises further advancements in science and medicine.